Category: Imaging
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Biology, Bessel beams and super resolution microscopy
Bessel beams are beam of laser light that are non-refracting and shaped liked the figure shown. The interesting characteristics about them are couple: 1.They do not diffract. Most light beams diffract and therefore do not make very good targets. 2.Their energy is spread over a larger area and therefore make great beams to work with…
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Luminscence based cell imaging and sensors – Nanolantern
In cell imaging, the most often used proteins are the Fluorescent proteins like (Green Fluorescent Protein}, GFP or many other fluorescent proteins like Yellow-FP, Blue-FP. They are popular because it is possible to express a labeled protein directly inside the cell. Before the discovery of fluorescent proteins, the only method was to express the protein,…
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Single molecule super resolution microscopy made simple
Bayes methods have come to microscopy. Researchers have developed the Bayesian analysis of photo-blinking and photo-bleaching in microscopy to deduce the structure that is not usually visible. (Bayesian localization microscopy reveals nanoscale podosome dynamics, Cox, S et al, Nat. Methods 9, 195 (2012)) http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.1812.html There have been many methods that are used for super resolution…
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Cloud computing for imaging.
Cloud computing is a very useful if you are processing large amount of data and cannot wait for your results or these are clinical and mission critical operations. However, there are several steps you need to understand before using it for imaging. The factors that affect most calculations for cost and use are: RAM: Random…
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Camera – Trillion frames a second.
Andreas Velten at MIT has been working in the lab of Ramesh Raskar to develop a camera that captures almost at the speed of light. This work was inspired by the bullet through the apple pictures by Doc Edgerton also at MIT. Their work is fascinating to see on Andreas website but it is hard…
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Computational Photography in Science
In the field of imaging there is another branch of image acquisition/analysis/study that is called Computational Photography as distinguished from traditional photography. In traditional photography, with a regular camera, you take a picture of a 3D scene through a camera. This light from the scene is captured as a 2-dimensional representation onto a photo-sensitive surface,…
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Shimmer microscopy – useful?
Microscopy has its origins from small lenses being put together for astronomy and then used for magnifying the small particles in a lake. Microscopy enabled the discovery of much what we know about cells, organelles and the function. It has also helped in the diagnosis of diseases – one of the earliest one being the…
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Imaging through rain.
Start with the application: A set of headlights that can enable the driver to see through the falling rain. Typically, it is difficult to see through rain because water droplets reflect the light that is reflected back from the water droplet. However, rain is not constant and it should be possible to time the light…
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Imaging: Precise real time Three dimensional Hand Mapping.
It is typically difficult to map in three dimensions (3D) at a very close distance in high precision very quickly. There are different ways to accurately represent three dimensions but that requires a 3D scanner that will take a number of “images” with light or infrared or sonar and then map it correctly. However, if…
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Photoacoustic imaging
It is difficult to image at high resolution using conventional optics to any reasonable depth below the skin. There are many ways to counteract this and one unique method is called photoacoustic imaging. This method was invented by Lihong Wang at Washington University in St Louis. The phenomenon of acoustic imaging is fascinating use of…