Tag: high resolution
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Bluesky: Diffraction images from flow cytometer
Flow cytometry typically flows particles or cells through a laminar flow cell into an interrogating laser beam to be read out as flourescent signal with a photomultiplier or photodiode. The read out is typically the integrated intensity or a two dimensional projection of a cell. The residence time is in order of 10 micro-seconds. Cellular…
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3D microscopy
Super resolution microscopy One of the problems with Quantum dots and some fluorescent proteins/molecules is that they blink. And they all photo-bleach. Bayesian analysis of blinking and bleaching or 3B microscopy analyzes images using Hidden Markov Model (HMM) allows useful information to be extracted from the data. Dr Susan Cox has come up with unique…