Category: Imaging
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Controls and standards
Controls and standards: Often times it is easy to get lost in the process of image quantitation rather than controlling the parameters. For example, it is easy to start writing the specifications of the camera, intensity of light, how many pixels per inch while having no control of what can be done. Next time doing…
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3D microscopy
Super resolution microscopy One of the problems with Quantum dots and some fluorescent proteins/molecules is that they blink. And they all photo-bleach. Bayesian analysis of blinking and bleaching or 3B microscopy analyzes images using Hidden Markov Model (HMM) allows useful information to be extracted from the data. Dr Susan Cox has come up with unique…
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Elastomer for surface imaging
Surface geometry made easy It is extremely difficult to measure the cell surface topology using microscopes or camera’s. Typically, the choice is to use reflected light, refracted light or some unique modification that requires light to enter at correct angles, be altered and then measured with the camera. Typically, setup like that cost hundreds to…
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Image processing to kill weeds
Not that it is difficult but if you have a garden and want to control weeds, the easiest method is to spray chemicals to kill the unwanted plants. More environmentally friendly measures take more time but it involves gardener’s sitting and picking up the unwanted plants. There are others who spray vinegar or acetic acid…
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Nanotube label free imaging
A researcher, Ji-Xin Cheng, at Purdue university has figured out a label free method to label carbon nanotubes. Nanotubes are typically hard to see and with Cheng’s technique not only can you see the nanotubes but you can also visualize them within cells. The method is known as transient absorbtion and uses a pulsed near…