Imaging

  • Controls and standards

    Byscientist

    Controls and standards: Often times it is easy to get lost in the process of image quantitation rather than controlling the parameters. For example, it is easy to start writing the specifications of the camera, intensity of light, how many pixels per inch while having no control of what can be done. Next time doing…

  • 3D microscopy

    Byscientist

    Super resolution microscopy One of the problems with Quantum dots and some fluorescent proteins/molecules is that they blink. And they all photo-bleach. Bayesian analysis of blinking and bleaching or 3B microscopy analyzes images using Hidden Markov Model (HMM) allows useful information to be extracted from the data. Dr Susan Cox has come up with unique…

  • Elastomer for surface imaging

    Byscientist

    Surface geometry made easy It is extremely difficult to measure the cell surface topology using microscopes or camera’s. Typically, the choice is to use reflected light, refracted light or some unique modification that requires light to enter at correct angles, be altered and then measured with the camera. Typically, setup like that cost hundreds to…